The effects of α7-specific ligands and DIDS on Ca²⁺ accumulation in mitochondria studied by flow cytometry.

A – purified liver mitochondria gated by size (Forward scatter) and granularity (Side scatter) in flow cytometry. B – binding of 0.1 µM acridine orange 10-nonyl bromide (NAO) to the gated mitochondria population; Control – the non-stained mitochondria. C – Ca²⁺ accumulation in mitochondria loaded with Fluo 3-AM. D – Ca²⁺ accumulated in mitochondria during 2 min after 1 min pretreatment with DIDS, acetylcholine, choline, PNU-282987 or MLA; data are shown as normalized mean fluorescence values of 5 independent experiments for each ligand; * – P<0.05 compared to the fluorescence in the absence of α7 nAChR agonists or DIDS.