The effect of NMDA is specific for mEPSC.

A. Histogram represents the number of mEPSCs as the function of the recording time (bin 10 s), NMDA is applied as indicated by the horizontal bar. Current traces represent average of events selected in control conditions between 0 and 50 sec (right), and at the peak of the increase of frequency induced by NMDA between 180 and 220 sec. Note that the two average currents display the same kinetic properties that fit with mEPSC (the trace on the left is more noisy because the average is constructed with few events). B. Left: mEPSCs recorded in control conditions (top trace) and after NMDA (bottom trace). Traces on the right (same cell), current trace in the presence of NBQX (top trace, mEPSCs are blocked) and after co-application of NMDA (bottom trace, no further effects of NMDA are detected).