The coiled-coil of human ATG16L1 is alpha helical.

(A) PSIPRED prediction of the secondary structure of the minimal coiled-coil domain (residues M126 – A207; CCD3) of human ATG16L1. Sequencing numbering corresponds to the human protein, alpha helices are marked as pink cylinders and the confidence level of the prediction for each residue is provided by the blue bars. (B) Circular dichroism analysis of CCD3. Mean residual ellipicity (θ) plotted against wavelength (nm) indicates a helical protein.