The cellular localization of S. pneumoniae GHIP.

Cells were grown in C+Y medium in presence of the indicated concentrations of ZnCl2 and harvested at mid-exponential growth for observation by automated phase-contrast time-lapse microscopy. (A) The fluorescence microscopy of strain R6 without fusion GHIP; (B) The C-terminal GFP fusion (GHIP-GFP); (C) The N-terminal GFP fusion GFP (GFP-GHIP).