The antioxidant behavior of Human Serum Albumin (HSA) in a pre-denaturation temperature thermal gradient.

The Accumulated Antioxidant Capacity (ACA%) appears as a function of the thermal change. Each point corresponds to an independent experiment verified at indicated temperatures. The antioxidant capacity (AC) of the albumin placed at 10°C was 48 nM TEU and corresponds to 100% of ACA%. From this point the antioxidant capacity of albumin increases with the temperature, until it reaches almost 200% ACA. RS correspond to ACA%-100 as specified in the Material and Methods section. Above 45°C it is possible to observe protein aggregation and light scattering at 400 nm in the stock solutions. Each point represents the average of three separate experiments ± SD. All points with a P<0.001 vs. 10°C temperature (ACA = 100%). *P<0.001 vs. previous and next point. ¹Temperature for aggregation is concentration-dependent (more details in the text).