The Rad53-kd variant restores DNA damage resistance and SSA in sae2Δ cells.

(A) Exponentially growing cells were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT or MMS. (B) DSB repair by SSA. The analysis was performed as described in Fig 4A. (C) Densitometric analysis of the product band signals. The experiment as in (B) was independently repeated three times and the mean values are represented with error bars denoting s.d. (n = 3).