The R2597 duplication is a TD in the chromosome.
(A) Predicted R2597 chromosome structure. A–E, duplicated regions; X/Z, flanks; Y, codY; R1/R2 flanking IS861; R2/1, predicted hybrid IS861. PCR detected the predicted junction in R2597. Green rectangle, TD junction; black rectangle, sequence represented in Figure 1B. (B) Representation of the R2/1 hybrid at the TD junction in R2597 identified by sequencing. Mismatches are represented by vertical black lines, extending either above (R1) or below (R2) the diagram. Horizontal green line represents region within which R1 and R2 recombined. Green arrows represent limits of recombination between R1 and R2. (C) PFGE analysis of R2597. Open arrows, restriction fragments predicted in Table S1. Red arrowhead, merodiploid-specific fragment revealed by hybridization. Although the lanes shown were part of the same initial gel/membrane, lanes present between them have been removed to simplify comparison between lanes.
