The Poc1acha/cha long bone growth plates become disorganized.
figure
posted on 2015-10-23, 03:05 authored by Krista A. Geister, Michelle L. Brinkmeier, Leonard Y. Cheung, Jennifer Wendt, Melissa J. Oatley, Daniel L. Burgess, Kenneth M. Kozloff, James D. Cavalcoli, Jon M. Oatley, Sally A. CamperA. Standard hematoxylin and eosin histological staining was carried out on paraffin sections from P0 and P15 tibial growth plates. Scale bar = 50 μm. B. Immunohistochemistry using a primary antibody against the Golgi marker GM130 or acetylated tubulin counterstained with DAPI in wild type and mutant P15 tibia sections. Scale bar = 25 μm. C. Immunohistochemistry for primary antibodies against the proliferation marker Ki67 (i) or cell death marker TUNEL (ii), with quantification of the number of TUNEL-positive cells per field of view in wild type and chagun tibia (iii). Scale bar = 100 μm.
