Figure_4.tif (264.94 kB)
The Cernunnos/XLF mutation lead to an increased activation of Chk1 in response to replication stress.
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posted on 2013-02-21, 06:58 authored by Michal Schwartz, Yifat S. Oren, Assaf C. Bester, Ayelet Rahat, Ruthy Sfez, Shlomo Yitzchaik, Jean-Pierre de Villartay, Batsheva KeremA. Cernunnos/XLF mutated and complemented cells were treated with 0.5 µM aphidicolin for 24 hours. Protein extracts were prepared and analyzed by immunoblotting with anti- pChk1(S317) antibodies. Untreated cells were analyzed as control. B. Quantification of pChk1(S317) levels in Cernunnos/XLF mutated and complemented cells following 0.5 µM aphidicolin treatment for 24 hours. pChk1(S317) level was normalized to Tubulin control.
