Test used at protein level to conclude on putative gene or putative pseudogene.

The species where the protein is tested is highlighted in bold type. Other species have known proteins. D1 and D2 are age of divergence, in millions of years. Note that all abbreviations concerning species name and their ancestors are provided in Figure 6 (A) The species in which the protein is analyzed is surrounded by species having different LCAs. If the sequence identity is higher between Mus and Hsa as compared to Mus and Mdo thus, we can conclude on a putative gene; otherwise on a putative pseudogene. (B) If all species with known protein have the same LCA as the species under investigation, a calculation step is necessary. Value1 is the percent identity between Mus and Hsa. Value2 is the percent identity between Mmu and Hsa. A minimum relative threshold is calculated by multiplying D2 distance by the similarity percentage Value2 and by dividing the total by the distance D. If the similarity percentage Value1 is superior to the minimum relative threshold calculated, we conclude on a putative gene; otherwise, we conclude on a putative pseudogene.