Tertiary and quaternary structures.

A) Circular dichroism spectra for GLB-26 compared with those for GLB-1 and horse heart Mb. The deconvolution analysis using the CDNN deconvolution software gave about 85% of α helical form for GLB-1 and Mb and slightly less (70%) for GLB-26. B) Molar masses measured by multi-angle light-scattering after gel filtration on a Superose 12 HR 10/300 GL column for GLB-26 (dotted line) and GLB-1 (dashed line) proteins. Relative absorbance at 280 nm is given in solid lines. The elution profile of GLB-26 in its ferric form is displaced toward the lower molar mass species and the width of the elution profile peak is broader than that for fully dimeric oxygenated GLB-1.