Taxonomy of Cupressaceae and Asteraceae and SDS-PAGE of purified allergenic proteins.
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posted on 2015-05-15, 04:16 authored by Ulrike Pichler, Michael Hauser, Martin Wolf, Maria Livia Bernardi, Gabriele Gadermaier, Richard Weiss, Christof Ebner, Hidenori Yokoi, Toshiro Takai, Alain Didierlaurent, Chiara Rafaiani, Peter Briza, Adriano Mari, Heidrun Behrendt, Michael Wallner, Fátima Ferreira(A) Phylogenetic tree of Cupressaceae and Asteraceae species. (B) Identity matrix of pectate lyase pollen allergens and, in case of Amb a 1, allergen isoforms. (C) SDS-PAGE of allergenic pectate lyases purified from pollen extracts by anion-exchange chromatography and/or size exclusion chromatography. The gel was stained with Coomassie Brilliant Blue R-250. Natural Cry j 1 migrates as visible double band.
