TUNEL staining in the retina of GPx4+/− and GPx4+/+ mice treated with NMDA or vehicle.
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posted on 2015-06-17, 03:17 authored by Osamu Sakai, Takatoshi Uchida, Murilo F. Roggia, Hirotaka Imai, Takashi Ueta, Shiro Amano(A) TUNEL staining of the retina after NMDA or vehicle treatment. Scale bar, 30 μm. (B) Comparison of the number of TUNEL positive cells in the retina. Data are mean ± SEM (n = 9–10). **p < 0.05.
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glutathione peroxidase 4tunelcells transfectedganglion cell layerLDH activity assayAnnexin V stainingR 28 cellsperoxidized lipid levelsgclGPx 4 expressionhneganglion cell damageprecursor cell line R 28Retina PurposeThe purposeGPx 4 siRNANMDA injection.ResultsGPx 4 knockdowncell densitycontrol siRNAincrease LDH activity
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