TUNEL and PH3 staining in A2ML ATG MO-injected embryos.

All embryos were at 5 dpf. A–D are transverse section, and E–F sagital section. A–B′ TUNEL staining of cont MO-injected embryo (A), and knock-down embryo (B). Areas in open square in A and B are magnified in the A′ and B′. C–D Confocal images of sections stained with anti-PH3^ser10 antibody. E–F PH3 staining in the intestine in cont MO (E) and in A2ML ATG MO-injected embryos (F). G Quantification of PH3-positive liver cells. Scale bars in (A and B) 20 µm, and in (C–F) 50 µm. es, esophagus; L, liver; y, yolk.