TGFβ-induced podocyte signaling involves small GTPases.

RT-PCR for TGFβ (A) in podocytes with and without exposure to stretch (24hours) in the absence or presence EHT (1µM) or SAR (1µM). (B) TGFβ protein expression detected by ELISA in podocytes with and without exposure to stretch (24hours) and treated with or without EHT (1µM) or SAR (1µM). Western blot for fibronectin (FN) (C) and quantification (D) produced by primary podocytes stimulated with or without TGFβ (10 ng/ml), in the absence or presence of EHT (1µM) or SAR (1µM). Annexin V staining after exposure with or without TGFβ (24h) (E) and its quantification (F) in podocytes treated with or without EHT (1µM) or SAR (1µM). Data represent the mean ± SEM of three independent experiments. *P<0.05 vs. corresponding non-stretched control (A, B) or non-stimulated control (D, F); # P<0.05 vs. stretched control (A, B) or TGFβ stimulated control (D, F).