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TEM analyses of recombinant P. pastoris under different physiological conditions showing differential peroxisome proliferation.

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posted on 2014-08-29, 02:58 authored by Arti Kumari, Rani Gupta

a) Control in BMMY medium 48 h, no peroxisome is visible; b) methanol fed culture 48 h, larger peroxisomes were observed; c) oleic acid fed culture 48 h, smaller and numerous peroxisomes were present; d) mixed fed culture (methanol + oleic acid) 48 h, peroxisome of varying size were observed; e) methyl oleate fed cultures after 24 h, larger peroxisomes few in number; f) methyl oleate fed cultures after 72 h, peroxisomes of varying size were observed, g) methyl oleate fed cultures after 96 h, numerous peroxisomes of varying size were observed. The magnification is 1 µm for all images. N  =  nucleus, V =  vacuole and P =  peroxisome.

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