TBP binding at the PHO5 promoter requires binding of Pho4 at UASp1.

ChEC analysis of TBP-MNase cleavage of PHO5 promoter DNA in the PHO5 wild type, and strains bearing mutations either in the TATA box or UASp1 of the PHO5 promoter. Autoradiographs show cleavage of PHO5 promoter DNA for PHO5 wild type (top), a TATA box mutant (middle), and a UASp1Δ mutant (bottom), respectively. Bands in the marker lane (M) indicate, from top to bottom, ScaI, BamHI, ClaI, and DraI cleavage, respectively.