Suppression of Nrf2 and NQO1 protein expression by cortisol in oxidative stress-induced H4IIE cells.

H4IIE cells were treated for 24 h with vehicle (DMSO), cortisone, sulforaphane or cortisone and sulforaphane in the presence or absence of H2O2. Cells were lysed, and equal protein amounts were used for Western blot analysis. Samples were probed for Nrf2 and NQO1 using actin as a loading control. The lower panel shows a densitometric analysis of Nrf2 (left) and NQO1 (right) protein normalized against β-actin. A representative experiment is shown.