Suppression of Nrf2 and NQO1 protein expression by cortisol in oxidative stress-induced H4IIE cells.

H4IIE cells were treated for 24 h with vehicle (DMSO), cortisone, sulforaphane or cortisone and sulforaphane in the presence or absence of H₂O₂. Cells were lysed, and equal protein amounts were used for Western blot analysis. Samples were probed for Nrf2 and NQO1 using actin as a loading control. The lower panel shows a densitometric analysis of Nrf2 (left) and NQO1 (right) protein normalized against β-actin. A representative experiment is shown.