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Suppression by the in vitro expanded CD8+CD28− T cells is restricted by donor HLA class I antigens.
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posted on 2013-02-20, 10:49 authored by Yuming Yu, Jennifer R. Zitzner, Josetta Houlihan, Nancy Herrera, Luting Xu, Joshua Miller, James M. Mathew, Anat R. Tambur, Xunrong LuoSuppression assays were set up as described in Figure 2, with the exception that the stimulator APCs for CD4+ proliferation were from either the priming donor (B-APC), an indifferent donor (I-APC, HLA-A, B and DR fully mismatched with donor B), or a partially matched donor (C-APC, who shared identical HLA class I, but not class II antigens, with donor B). Proliferation of the CD4+ cells was measured by CFSE dilution. Data shown are representative of three independent experiments using three pairs of donors B, C and I. Table 1 provides one example of the HLA class I and class II antigen typing of individuals B, C, and I used for one of three such experiments.