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Subcellular localization of TDE2508.

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posted on 2014-02-21, 03:18 authored by Yuki Abiko, Keiji Nagano, Yasuo Yoshida, Fuminobu Yoshimura

The whole cell lysate (WCL), soluble fraction (Sol) and envelope fraction (Env) of T. denticola ATCC 35405 were separated by SDS-PAGE with CBB-staining (A) and analyzed by Western blotting with anti-TDE2508 antiserum (B). The envelope fraction was further fractionated by differential solubilization in 0, 0.25, 0.5, 1, 2, 4, and 8% Triton X-100, and the soluble fractions (lanes 1–7, respectively) and insoluble fractions (lanes 8–14, respectively) were subjected to Western blot analysis with anti-T. denticola whole cell antiserum (C) and anti-TDE2508 antiserum (D). The grey, white and black arrowheads denote Msp, TmpC and TDE2508, respectively. M denotes a standard marker.

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