Figure_5.tif (641.47 kB)
Subcellular localization of OfHex3 in the insect cell line Sf9.
figure
posted on 2013-08-12, 01:48 authored by Mingbo Qu, Tian Liu, Peng Chen, Qing YangOfHex3 was expressed in insect cell line Sf9 fused with N-terminal V5 tag. It was co-transfected with Golgi marker Man II-GFP. The subcellular localizations of the recombinant proteins were detected through immunofluorescence using anti-V5 antibody. The red signal indicates recombinant protein and the green signal indicates Man II-GFP in merge.
