Structures and UV-Vis absorption spectra of NEET proteins and Anamorsin.
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posted on 2015-10-08, 03:49 authored by Colin H. Lipper, Mark L. Paddock, José N. Onuchic, Ron Mittler, Rachel Nechushtai, Patricia A. JenningsA. (Top) Crystal structures of mNT (PDB code: 2QH7,), NAF-1 (PDB code: 3FNV); (Middle) NEET 2Fe-2S cluster with 3-Cys:1His coordination; (Bottom) Absorption spectra of 25 μM mNT and NAF-1. B. (Top) Crystal structure of the N-terminal domain of Anamorsin (PDB code: 2YUI) with an added schematic of the unstructured 2Fe-2S cluster binding domain; (Middle) Representative Anamorsin 2Fe-2S cluster with 4-Cys coordination (from ferredoxin, PDB code: 1RFK); (Bottom) Absorption spectrum of 43 μM Anamorsin isolated from E. coli.
