Structure specificity and neutralizing activity of anti-Cyr61 antibody.

(A) Western blot of 50 ng recombinant CTGF and Cyr61 protein detected by an anti-Cyr61 antibody. This anti-Cyr61 antibody could specifically recognize Cyr61 but not structurally relevant CTGF. (B) Culture medium containing 400 ng/mL of recombinant Cyr61, pretreated with 50 µg/mL anti-Cyr61 antibody or control rabbit IgG for 1 hour, were added to NRK-52E cells for 2 hours. Q-PCR showed a significant reduction in Cyr61-enhanced MCP-1 expression after anti-Cyr61 antibody pretreatment. N = 4/group. (C) The NRK-52E cells were treated with 1 ng/mL of TGF-β1 and 50 µg/mL of anti-Cyr61 antibody or control rabbit IgG for 24 hours. Q-PCR showed that anti-Cyr61 antibody attenuated TGF-β1-induced MCP-1 upregulation. N = 4/group. The values are the mean+SD. *P<0.05.