Stimulation of T cells with PMA/ionomycin and/or NiV infection.

Figs. 5.A–5.D illustrate changes in cell appearance of the CD6+ T cells after 18 hrs post infection with NiV at 0.1 moi. Formation of cell clusters in NiV infected cells suggests that the infection stimulates T lymphocytes. Appearance of non-stimulated T cells (Fig. 5.A) in comparison to NiV infected T cells (Fig. 5.B) and the PMA/ionomycin stimulated cells (Fig. 5.C). NiV infected PMA/ionomycin activated T cells (Fig. 5.D). Fig. 5.E Cytokine RNA profiles were determined 48 hrs post incubation/infection by quantitative RT-PCR. Gray columns represent ratio of PMA/ionomycin activated cells and non-stimulated T cells; white columns represent ratio of NiV infected cells compare to non-stimulated cells; black columns represent ratio of infected PMA/ionomycin activated cells compared to PMA/ionomycin activated T cells.