Figure_4.tif (273.72 kB)
Stability of SULT4A1 protein in IMR-32 cells.
figure
posted on 2014-07-02, 03:04 authored by Neelima P. Sidharthan, Neville J. Butcher, Deanne J. Mitchell, Rodney F. MinchinCells were transfected with plasmids for FLAG-tagged wild-type or variant SULT4A1 and then treated with 10 µg/ml cycloheximide for the indicated times. Proteins were then Western blotted using anti-FLAG antibody and anti-tubulin antibody as a loading control. Data are normalized to protein levels at time 0. All results are mean ± s.e.m, n = 4.