Spectrosome material is transferred via ring canal in GSCs.

Spectrosome (Short Adducin-GFP) in wild type GSC is located in the basal position (0 min: interphase and 24min: metaphase). At 1hr 12min, spectrosome is co-localized with the ring canal structure. Spectrosome, together with ring canal structure, grows into the stem cell daughter at 6hr 16min, segregates into both daughter cells and form spectrosome-like structure again at 15hr 20 min. At 20hr 40 min, spectrosomes in both GSC and gonialblast further develop, and the gonialblast separates from GSC. Cellular boundaries and mitotic spindle are visualized with α-tub-GFP. Black arrow: spectrosome. White dash-arrow: spectrosome and/or ring canal tail. *: hub cells. Yellow dash-line: GSC or gonialblast boundary. Solid white arrow: mitotic spindle.