SmHsf1 can drive transcription in a modified yeast one-hybrid system.

Yeast cells expressing SmHsf1 fused to the Gal4 DNA binding domain (Gal4DBD-SmHsf1) were patched (A) or serially diluted (B and C, from 1 to 10⁻⁵) on different selective media to test the ability of SmHsf1 to activate transcription. The positive control yeast express a complete GAL4 gene (Gal4Full) and the negative control yeast express the GAL4 DNA binding domain alone (Gal4DBD). (A) Blue color on SD-Trp with X-α-Gal indicates expression of the MEL1 reporter gene. (B and C) Growth on the SD-His and SD-Ade plates indicates expression of the HIS3 and ADE2 reporter genes, respectively, and are essential for cell viability.