SjE16.7 induces neutrophil migration in vitro.

(A) Cell migration was measured using transwell assay. Neutrophils were plated in the upper chamber of the filter that had been coated with fibronectin, and stimulated with denature His-SjE16.7 (95°C, 30 min treatment) or His-SjE16.7 as indicated concentration for 3 h. Cells migrating to the bottom chambers were counted. Soluble egg antigen (SEA, 10 µg/ml) was used as control. Values are means ± S.E.M of 4 independent assays. **P<0.01 vs. group without antigen stimulation. (B) Transwell assays were performed as described in A, except that cells were stimulated with prokaryotic protein GST or GST-SjE16.7 as indicated. Values are means ± S.E.M of 3 independent assays. **P<0.01 vs. group without antigen stimulation. (C) Transwell assays were performed as described in A, except that cells were stimulated with recombinant SjE16.7 with or without purified anti-SjE16.7 antibody (10 µg/ml) as indicated. Values are means ± S.E.M of 2 independent assays. **P<0.01. (D) Transwell assays were performed as described in B, cells were stimulated with recombinant GST or GST-SjE16.7 with or without Polymyxin B (10 µg/ml) as indicated. Values are means ± S.E.M of 2 independent assays. **P<0.01 vs. group without antigen stimulation.