Sequencing chromatogram.

The sequencing chromatogram shows two nearby ambiguous sequence positions 610 and 612. At position 610 adenine and guanine are present. At position 612 adenine and thymine are present. Positions are numbered with respect to the Reverse Transcriptase of the hepatitis B virus genome. This chromatogram raises the question which of the bases at positions 610 and 612 are present on the same clonal variant.