Sensitization and treatment protocol.

Mice in the AR, positive, and negative groups were sensitized with 100 μg of ovalbumin (OVA) and 1 mg of aluminum hydroxide on days 0, 7, and 14. On days 21–35, these mice were intranasally administered 40 μl of saline containing 400 μg of OVA (20 μl per nostril) for secondary immunization. Furthermore, within 3 h of each treatment on days 21–35, the AR group was treated with saline, whereas the positive and negative groups were nasally administered 2 × 10⁶ infectious units (IFUs) of LV miR-135a and 2 × 10⁶ IFUs of an empty LV vector, respectively.