Secondary structure and disorder predictions of the MNV NS1-2 protein.
(A) MeDor output showing five different disorder predictors with regions of disorder indicated by bi-directional arrows (IUPred – red, GlobPlot2 – black, DisEMBL – green, FoldIndex – brown, RONN – purple). CASP119, caspase 3 cleavage site. (B) PONDR® graph showing predicted disordered and ordered segments. The strength of the prediction is indicated by the PONDR® score on the y-axis. Regions above 0.5 are considered disordered [36] and are indicated by a solid black line through the central x-axis, with the corresponding average strength shown in the attached box. (C) PSIPRED secondary structure prediction. Pink barrels indicate helices, yellow arrows indicate strands, and the strength of the prediction is shown as the blue graph above the structural prediction (D) Kyte-Doolittle hydropathy plot. Hydrophobic regions are indicated above the x-axis. TM, putative transmembrane domain (residues 266–318) predicted by PSIPRED.
