Schematic representation of blood samples analysis process.
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posted on 2014-04-07, 03:56 authored by Aleksandra Markiewicz, Magdalena Książkiewicz, Marzena Wełnicka-Jaśkiewicz, Barbara Seroczyńska, Jarosław Skokowski, Jolanta Szade, Anna J. ŻaczekCollected blood sample was layered on density gradient. After centrifugation fraction containing tumor cells (marked by green box) was collected and subjected to negative selection. After CD45-depletetion, CTC-enriched blood sample was subjected to RNA isolation, reverse transcription and gene expression analysis with qPCR.
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anatomyCardiovascular anatomycell biologyMolecular cell biologygeneticsCancer geneticsgene expressionphysiologyCardiovascular physiologyBlood circulationepidemiologyCancer epidemiologyoncologyBasic cancer researchmetastasisCancers and neoplasmsBreast tumorsresearch designClinical research designsamples
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