Schematic of the study design. A.

Paired microarray of normal and lymphedematous skin derived from 27 subjects with lymphedema of one or more limbs. RNA was isolated from the whole tissue. Pathway analysis was performed to identify the final targets for protein analysis. B. High throughput assay of identified targets was performed using the Luminex 51-plex bead assay. Assays were performed on the original 27 lymphedema subjects and on 12 healthy normal controls. Logistic regression modeling was performed to identify the final targets for prospective analysis. C. Prospective assay for the 6 target proteins was performed on a distinct cohort of 36 lymphedema subjects and 15 normal controls. The results were analyzed through plotting a receiver operating characteristic (ROC) curve.