Samples with increased TRA-1-60+ve cells from patients with CIN3 have enhanced expression of stem cell proteins.
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posted on 2014-12-22, 02:41 authored by Maurice Canham, Chara Charsou, June Stewart, Sharon Moncur, Laura Hoodless, Ramya Bhatia, Duanduan Cong, Heather Cubie, Camille Busby-Earle, Alistair Williams, Victoria McLoughlin, John D. M. Campbell, Kate Cuschieri, Sarah HowieA) Flow cytometric analysis of samples used for proteomic microarray. Dotted lines show that the three samples with a significant increase in TRA-1-60 +ve cells have similar levels of cycling cells to the other three CIN3 samples. * = p<.05. B) Image-J pixel density analysis of stem cell proteomic microarray. SOX2, TP63 and HCG are further increased in samples with increased TRA-1-60+ve cells. C) Inverted image of the scan of the array from TRA1-60+ve samples; D) Inverted image of the scan of the array from TRA1-60 –ve samples.
