SPX has little effect on changes in the pancreatic islets in IL-10-deficient mice.

(A) Representative images of insulin staining (left), Mallory-Azan staining (middle), and α-SMA staining (right) in pancreas sections from each group. Scale bar = 100 µm. (B−F) Insulin-staining area in the pancreas (B), intra-islet fibrosis area (C) and intra-lobular fibrosis area (D), hydroxyproline content (E) and α-SMA-positive cells (F) in each group (n = 6). *P<0.05 vs. the Sham group (wild-type or IL-10KO mice), #P<0.05 vs. SPX mice (wild-type). Treatment groups: Sham, fed a HF diet, given serum albumin and sham-operated; SPX, fed a HF diet, given serum albumin, and splenectomized; SPX+IL-10, fed a HF diet, given recombinant mouse IL-10 and splenectomized. Wild-type, wild-type mice; IL-10KO, IL-10-deficient mice.