SDS-PAGE analysis of secreted RKOD in the cell culture supernatants of the shake flasks and soluble KOD and KOD-S expressed in E. coli.
figure
posted on 2015-07-02, 02:57 authored by Fei Wang, Shuntang Li, Hui Zhao, Lu Bian, Liang Chen, Zhen Zhang, Xing Zhong, Lixin Ma, Xiaolan Yu(A). M, protein molecular weight marker (the size of each band is indicated on the left); lane 1, cell culture supernatant (15 μl) of the strain bearing the pHBM905A plasmid (negative control); lanes 2–7, cell culture supernatants (15 μl) collected from days 1–6, respectively; and lane 8, purified RKOD; (B). M, protein molecular weight marker (the size of each band is indicated on the left); lane 1, purified KOD; lane 2, purified KOD-S.
