Figure_3.tif (2.88 MB)
Retinal reconstitution assay.
figure
posted on 2012-08-03, 01:58 authored by Rosana Simón-Vázquez, Marta Domínguez, Víctor A. Lórenz-Fonfría, Susana Álvarez, José-Luís Bourdelande, Ángel R. de Lera, Esteve Padrós, Alex Perálvarez-MarínSpectra of the UV-Vis absorbance of the WT and T90A bOp after incubation with all-trans retinal and 13-ethyl retinal in 50 mM phosphate buffer at pH 6.5. The bOp concentration is 1.0×10−5 M and the retinal was added to have a molar ratio 1∶1.5 bOp/retinal. The band at 380 nm (*) is related with the absorbance of the free retinal. The bands that appear in the region of 500–600 nm (**) correspond to the retinal that has reached the RBP of the corresponding bOp and formed a protonated SB.
