Results of the immunoblotting analysis and TRAP assay.
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posted on 2015-12-01, 15:02 authored by Kengo Kuroda, Tohru Kiyono, Emiko Isogai, Mizuki Masuda, Moe Narita, Katsuya Okuno, Yukako Koyanagi, Tomokazu Fukuda(A) Detection of CDK4 and cyclin D1 by immunoblotting. Lane 1, BFCE primary cells; Lanes 2 and 3, BFCE-K4DT cells. (B) Detection of telomerase activity by TRAP assay. Lane 1, 1 kb ladder marker; lane 2, CHAPS buffer as a negative control; lane 3, 293T cells as a positive control; lane 4, BFCE primary cells; lane 5 and 6, BFCE-K4DT cells. The 6-bp ladders were detectable in lanes of positive control and BFCE-K4DT cells. Each experiment was performed in duplicates after cloning (primary cells: 2 times passage, K4DT cells: 15 times passage) and representative data were shown here.
