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Representative 2-DE map of control A375 melanoma cells.

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posted on 2015-07-15, 05:29 authored by Lucia Cattaneo, Rosella Cicconi, Giuseppina Mignogna, Alessandra Giorgi, Maurizio Mattei, Giulia Graziani, Rosalia Ferracane, Alessandro Grosso, Patrizia Aducci, M. Eugenia Schininà, Mauro Marra

Proteins were electrophoretically separated in the non-linear pH range 3–10 and the 200–15 kDa molecular mass range and visualized by colloidal Coomassie staining. The encircled spots indicate the proteins affected by rosemary extract (1:240; 24h) treatment. The relative intensities, between control and treated samples, are shown in panels. Data significance was evaluated by a Student’s t-test (P ≤0.05). PDA3, protein disulfide-isomerase A3; GANAB, Neutral alpha-glucosidase AB; PCBP1, poly(rC)- binding protein 1; PCBP2, poly(rC)-binding protein 2; LMNA, lamin A.

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