Recruitment of IcmSW to the membrane by the dotL mutants.

Subcellular localization of IcmS was assayed using L. pneumophila lysates separated into total (T), soluble (S) and membrane (M) fractions. ΔA ΔL is the ΔdotA ΔdotL double mutant and all of the dotL mutants were expressed in a ΔdotL background. Protein fractions were analyzed by western blot using an IcmS-specific antibody. Fractions were loaded proportionally and are representative of three independent experiments.