Figure_4.tif (994.16 kB)

Real-time RT-PCR validation for selected probe sets.

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posted on 2013-12-03, 03:55 authored by Chunchao Wang, Qiang Wei, Kang Zhang, Ling Wang, Fengxia Liu, Linna Zhao, Yuanjun Tan, Chao Di, Hong Yan, Jingjuan Yu, Chuanqing Sun, Wenqiong J. Chen, Wenying Xu, Zhen Su

Twelve genes were selected for real-time RT-PCR to validate the expression patterns of Ubi::OsSPX1-antisense transgenic lines (A1 and A2) and WT rice (Ni) seedlings under control condition and cold treatment. These genes are: LOC_Os05g48390 - OsPHO2, ubiquitin conjugating enzyme; LOC_Os10g38600 - glutathione S-transferase GSTU6; LOC_Os10g38540 - glutathione S-transferase GSTU6; LOC_Os10g38350 - glutathione S-transferase GSTU6; LOC_Os01g72150 - glutathione S-transferase; LOC_Os01g72140 - glutathione S-transferase; LOC_Os01g43774 - cytochrome P450 CYP72A23; LOC_Os07g23570 - cytochrome P450 CYP709C9; LOC_Os09g25070 - OsWRKY62; LOC_Os02g08440 - OsWRKY71; LOC_Os06g37300 - ent-kaurene oxidase; LOC_Os11g47500 - xylanase inhibitor protein 1 precursor. The error bars of real-time RT-PCR results represent the standard error from three independent biological replicates.