RAW 264.7 cells vector migration ability.

A) Schematic of the optical microscope with microfluid technology for assessing macrophage vector migration ability. B) RAW264.7 cell mobility changed after NP uptake. Cell movement was followed by live confocal microscopy for 24 h. The origin of each cell is at the intersection of the x- and y-axis, 20 cells were assessed per experiment (performed in triplicate). Cell migration over a defined area was determined in fresh media in the control group vs. pre-treatment with 30-, 50-, 100-, and 500-nm NPs, respectively. The results were analyzed, and the histograms illustrate the trajectory C) and vector D) rate of RAW264.7 cells following different pre-treatments. *p<0.05 compared with control (Error bar is SEM).