RADA promotes increased survival of hamsters inoculated with 263K Scrapie.
Fig. 1A compares hamster survival in days (mean±SEM) following intracerebral inoculation with 1% Scrapie alone (PrPsc), Scrapie pre-incubated with 0.9% w/v RADA (PrPsc+RADA) or Scrapie agarose plugs (PrPsc+agarose). Fig. 1B compares hamster survival in days (mean±SEM) with increased dilution of Scrapie brain homogenate alone (open squares) to equivalent doses of Scrapie pre-incubated with 0.9% RADA (open circles). Fig. 1C depicts a hamster survival curve in days (N = 6; mean±SEM) with increasing dilution of Scrapie inoculant (open circles). Plotted on the curve (open square) is the mean survival in days of hamsters inoculated with 10−2 Scrapie combined with 0.9% RADA depicting equivalent titer of Scrapie inoculum. Fig. 1D shows a dose-dependent increase in hamster survival in days (mean±SEM) with increasing concentration of RADA (open circles) inoculated with 1% Scrapie. A synthetic 16-mer RADA-peptide was hydrated combined with 1% Scrapie and inoculated. Survival in days was plotted (grey triangle) and used to estimate RADA concentration.
