RAD001 and BEZ235 synergistically inhibit growth of 786-O cells in vitro and in vivo.
786-O cells (PTEN and VHL-deficient renal cell carcinoma) were treated for 72 h with different doses of RAD001 and/or BEZ235, inhibition of cell viability was measured using CellTiter-Glo (A), and Chalice software was used to calculate excess inhibition over Loewe additivity for each RAD001 and/or BEZ235 dose combination (B). Data values represent the mean from 3 wells. Mice bearing 786-O xenografts were dosed daily for 3 weeks with RAD001, BEZ235, or the combination of the two compounds. Mean tumor volumes were measured and are plotted as a function of time (C).