Figure_1.tif (937.07 kB)
Quaternary structure in solution.
figure
posted on 2011-10-03, 02:25 authored by Charlene Willis, Conan K. Wang, Asiah Osman, Anne Simon, Darren Pickering, Jason Mulvenna, Alan Riboldi-Tunicliffe, Malcolm K. Jones, Alex Loukas, Andreas HofmannLeft: Size exclusion with MALS detection reveals monomeric Na-SLP-1 and dimeric Ac-SLP-1 at pH 7.5 and 5.0 under normal salt conditions (representative chromatograms shown). Right: Ac-SLP-1 is exclusively observed as a dimer on native gels. The protein migrates at 20 kDa under native conditions (Lane 1), but at smaller sizes in the presence of SDS (Lane 2). The migration behaviour of Na-SLP-1 on native gels does not allow analysis due to smeared bands (data not shown).
