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Quantitative analysis of UCP2 expression in tissues using the recombinant proteins UCP2 and UCP1.

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posted on 2012-08-03, 01:59 authored by Anne Rupprecht, Anja U. Bräuer, Alina Smorodchenko, Justus Goyn, Karolina E. Hilse, Irina G. Shabalina, Carmen Infante-Duarte, Elena E. Pohl

(A) The comparison of UCP2 amounts in different tissue samples and isolated cells. UCP2 was calculated in ng of µg total protein in whole tissue (cell) lysate. Each data point represents the mean ± SE of six mice. (B) Representative Western Blot showing UCP2 quantification in thymus. (C) Representative Western Blot of UCP1 protein amount compared to the total cell protein in BAT.

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