Figure_7.tif (502.52 kB)
Quantitation of the DSB-2 positive zone in WT and meiotic mutants.
figure
posted on 2013-08-08, 02:43 authored by Simona Rosu, Karl A. Zawadzki, Ericca L. Stamper, Diana E. Libuda, Angela L. Reese, Abby F. Dernburg, Anne M. VilleneuveBar graph showing the extent of the region of DSB-2 positive nuclei in germ lines of indicated genotypes. The presence/absence of DSB-2 signals was assessed in the portion of the germ line extending from the onset of DSB-2 staining to the end of the pachytene region. The extent of the DSB-2 positive zone was defined as the percentage of continuous rows of nuclei in which all or most nuclei exhibited DSB-2 staining out of total rows of nuclei in the scored region. Representative germ lines were imaged and scored: 5 for WT and 3 for each of the meiotic mutants. Error bars show standard deviation.