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Quantitation of H3K4me3 and H3K27me3 levels in response to c-Myc.

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posted on 2009-11-13, 01:55 authored by Chin-Hsing Lin, ChenWei Lin, Hisashi Tanaka, Matthew L. Fero, Robert N. Eisenman

(A) Response of H3K4me3 to Lentiviral mediated overexpression or knock-down c-Myc in mES cells as determined by western blot. (B) Response of H3K27me3 to Lentiviral mediated overexpression or knock-down c-Myc in mES cells as determined by western blot. (C) Effect of H3K4me3 and H3K27me3 level in response to c-Myc loss in early developmental embryos. Ratios based on normalization to γ-tubulin internal control wwere calculated using ImageJ software .

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