Protein expression profile and differentiation of the primary and 5^th passaged BM-MSCs toward cardiocytes and vascular endothelial cells in vitro.

(A) The representative images of silver stained 2-DE gel of the proteins from the primary pour-off cultures (upper panel, 1^st BM-MSCs) and the 5^th passaged cultures (low panel, 5^th BM-MSCs). (B) Desmin, (C) Troponin I, (D) MHC-ß, (E) VEGF, (F) FLT-1 and (G) KDR mRNA levels were examined in BM-MSCs from the primary pour-off cultures (1^st BM-MSCs) and the 5^th passaged cultures (5^th BM-MSCs) induced by 5′-azacytidine (B–D) and VEGF (E–G) by real-time RT-PCR. The mRNA levels were calculated as a ratio to the GAPDH mRNA level and expressed relative to levels of the 1^st BM-MSCs. Each value was the means±S.E.M. of 5 determinations. **: P<0.01; ***: P<0.001 compared with the 1^st BM-MSCs.