posted on 2014-06-10, 03:24authored bySeunghee Cho, Ke Shi, Jennifer L. Seffernick, Anthony G. Dodge, Lawrence P. Wackett, Hideki Aihara
After substrate binding, Ser226 attacks the substrate carbonyl, producing an tetrahedral intermediate. Arginine and a backbone amide group form an oxyanion hole to stabilize the negative charge on intermediates. The substrate carbonyl reforms with the cleavage of the C-N ring bond. Water then attacks the acyl intermediate to release the product.